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This system involved the administration of anabolic steroids on rats, either orally or by injection (depending on the anabolic steroid being assessed)during the early embryonic and juvenile growth phases. A total of 1,800 rats were used in this study. The rats were maintained on standard rat chow under controlled conditions until the third day of gestational age. The rats were administered either an oral concentration of anabolic steroids or the injection of anhydroperoxylated acesulfame K (a single bolus dose equal to 5 mg/kg body weight) at doses equivalent to 10-20 mg/kg body weight. The anabolic steroid doses for each group were chosen to achieve the highest anabolic steroid serum levels in each rat. The rat experiments were performed at the Department of Pharmaceutical Sciences, University of California, Irvine School of Medicine. The animals were maintained on a water-supplied, 8-h overnight chow. During the first 24 h and immediately prior to the injection, the rats performed a food deprivation protocol. Following the injection of the anabolic steroids, a 2-h period was observed for food intake and body fluid sampling. Body weights of all rats were measured, and mean weights at 12 and 72 h after the injection were compared via the Dunnett's Multiple Range Test (Dunnett, 1959 ). After the 2-h water-supplementation period and before receiving injections, the animals were anesthetized with pentobarbital (50 mg/kg), or pentobarbital 25 mg/kg (pint), followed by isoflurane (100 mg/kg), and then the rat's head was shaved. The head was then removed and the body dissected out. All analyses followed established laboratory procedure with minimal alteration of the experiments. After removal of the decapitated head, a blood sample was taken to determine the anabolic steroid concentration in the brain as a function of time. These measures were used to establish time-by-group effects in the anabolic steroid studies. In the present study, we utilized a previously published animal model to study the potential effects of aldosterone and anabolic steroids alone or in conjunction on the reproductive system of rats. Male Sprague-Dawley rats (1.5–2.5 kg, 19–25 in weight) were obtained from Charles River (Rochester, MN) and kept from 0.25 to 1.5 weeks of age. Immediately before injection with anhydroperoxylated acesulfame K, the animals received their normal chow. On day 2 of gestational age, the rats were assigned to a 1, Similar articles: